Anda belum login :: 03 Oct 2022 14:42 WIB
ArtikelA reduced oxygen tension (5%) is not beneficial for maintaining human embryonic stem cells in the undifferentiated state with short splitting intervals  
Oleh: Hsin-Fu, Chen ; Hung-Chih, Kuo ; Wannhsin, Chen ; Fang-Chun, Wu ; Yu-Shih, Yang ; Hong-Nerng, Ho
Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Human Reproduction vol. 24 no. 01 (Jan. 2009), page 71.
Topik: embryonic stem cells; hypoxia; undifferentiated; oxygen; cell culture
  • Perpustakaan FK
    • Nomor Panggil: H07.K.2009.01
    • Non-tandon: 1 (dapat dipinjam: 0)
    • Tandon: tidak ada
    Lihat Detail Induk
Isi artikelBACKGROUND: Human embryos grow naturally in vivo in lower oxygen (O2) tension environments than atmospheric O2 tension. Therefore, human embryonic stem cells (hESC), a derivative of embryos, will likely grow more favorably in a reduced O2 tension. This study aimed to compare the behavior of hESC under reduced O2 tension (5%) versus normoxia (21%). METHODS: hESC lines were cultured in different O2 tensions and then examined for morphology, apoptosis and gene expression profiles. RESULTS: hESC grown in 5% O2 tension were not morphologically different from hESC grown in normoxia on day 7 of the first and fourth passages. However, after prolonged culture without splitting (10–14 days), hESC colonies were thinner and looked better morphologically in 5% O2, but the cells proliferated more slowly and their sizes were larger. At most time points, the gene expression profiles in both O2 tensions showed no major difference in representative stemness genes (Oct-3/4, Nanog and Cripto), differentiation genes (Desmin, Nestin, -fetoprotein and GDF-9) and hypoxia-related genes (HIF-1 and VEGF). A lower level of cyclin-D1 mRNA (suggestive of less Wnt pathway signaling on day 7 of the fourth passage) and a higher level of Desmin (suggestive of more differentiation to mesoderm, at day 7 of the first passage) were detected in 5% O2. CONCLUSIONS: This study suggests that for routine culture of hESC with a short splitting interval (7 days), a low O2 tension (5% O2) probably does not provide significant advantages over the standard 21% O2 tension for the maintenance of an undifferentiated state by the criteria used in this study.
Opini AndaKlik untuk menuliskan opini Anda tentang koleksi ini!

Process time: 0.0156271 second(s)