Anda belum login :: 03 Oct 2022 14:42 WIB
A reduced oxygen tension (5%) is not beneficial for maintaining human embryonic stem cells in the undifferentiated state with short splitting intervals
Article from Journal - ilmiah internasional
Human Reproduction vol. 24 no. 01 (Jan. 2009)
embryonic stem cells
1 (dapat dipinjam: 0)
Lihat Detail Induk
BACKGROUND: Human embryos grow naturally in vivo in lower oxygen (O2) tension environments than atmospheric O2 tension. Therefore, human embryonic stem cells (hESC), a derivative of embryos, will likely grow more favorably in a reduced O2 tension. This study aimed to compare the behavior of hESC under reduced O2 tension (5%) versus normoxia (21%). METHODS: hESC lines were cultured in different O2 tensions and then examined for morphology, apoptosis and gene expression profiles. RESULTS: hESC grown in 5% O2 tension were not morphologically different from hESC grown in normoxia on day 7 of the first and fourth passages. However, after prolonged culture without splitting (10–14 days), hESC colonies were thinner and looked better morphologically in 5% O2, but the cells proliferated more slowly and their sizes were larger. At most time points, the gene expression profiles in both O2 tensions showed no major difference in representative stemness genes (Oct-3/4, Nanog and Cripto), differentiation genes (Desmin, Nestin, -fetoprotein and GDF-9) and hypoxia-related genes (HIF-1 and VEGF). A lower level of cyclin-D1 mRNA (suggestive of less Wnt pathway signaling on day 7 of the fourth passage) and a higher level of Desmin (suggestive of more differentiation to mesoderm, at day 7 of the first passage) were detected in 5% O2. CONCLUSIONS: This study suggests that for routine culture of hESC with a short splitting interval (7 days), a low O2 tension (5% O2) probably does not provide significant advantages over the standard 21% O2 tension for the maintenance of an undifferentiated state by the criteria used in this study.
Klik untuk menuliskan opini Anda tentang koleksi ini!
Copyright © 2006, 2007
Unika Atma Jaya
, all rights reserved
Process time: 0.0156271 second(s)