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BukuAntibiofilm Activity of Bioactive Compound From Pseudomonas stutzeri AF3 Against Pathogenic Bacteria (presented at ASM Microbe meeting 2016, Boston, USA)
Author: Waturangi, Diana Elizabeth ; Suteja, Lisda ; Suhartono, Maggy Thenawidjaja
Topik: Biofilm; Antibiofilm; Pseudomonas stutzeri; JABFUNG-DW-GB-2018-05
Bahasa: (EN )    
Penerbit: American Society of Microbiology (ASM)     Tempat Terbit: Boston, USA    Tahun Terbit: 2016    
Jenis: Papers/Makalah - pada seminar internasional
Fulltext: Antibiofilm activity Pseudomonas AF3.pdf (4.56MB; 10 download)
Biofilm is an assemblage of surface-attached microorganism embedded in exopolysaccharide (EPS) matrix. It is one of the key factor for microbial survival in adverse environment. Biofilm not only enhance cell-to-cell communication but also protect the cells from desiccation, radiation, and damaging agent such as oxidizing molecules and antibiotics. Therefore, it poses a major threat to industrial, environmental, and medical field. In the recent years, various means have been explored in order to control biofilm formation and accumulation. Many recent studies suggest that antibiofilm compounds extracted from diverse microorganism have the ability to control biofilm without exhibiting biocidal activity (Kostakiotiet al. 2015).
From previous studies, endophytic bacteria AF3 (Pseudomonas stutzeri) had been isolated from Anrederacordifolia and had been shown antibiofilm activity against wide range of human pathogenic bacteria (Jessica 2011; Febri 2013). In this study we purified the compound from Pseudomonas stutzeri AF3 using ion exchange chromatography as well as desalting to concentrated the sample followed by antibiofilm assay using static biofilm assay to inhibit and destruct biofilm formation of Pseudomonas aeruginosa and Staphylococcus aureus.
The compound was purified using ion exchange chromatography showed single peak when eluted using linear gradient of NaCl 2 M. While desalting was done to obtain concentrated sample.. Significant increase in antibiofilm activity of purified samples was observed. The highest increase observed in inhibition and destruction antibiofilm of P. aeruginosa at 10% concentration of sample. Concentrated sample exhibited relatively high antibiofilm activity when compared with purified sample even after 10-fold dilution. There was an exception for biofilm inhibition against S. aureus where the antibiofilm activity of concentrated sample diluted 10-fold was much lower than purified sample. The highest increase in biofilm inhibition activity was showed against P. aeruginosa when 5% of compound was added whereas in destruction activity 10% of compound added to P. aeruginosa showed highest increase. Bioactive compound from Pseudomonas stutzeri showed antibiofilm activity agains P. aeruginosa and S. aureus, the activity increased after purification. This suggested that polysaccharide might have an important role in biofilm destruction activity of this compound.
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