Detail Metabolic engineering of plant alkaloid biosynthesis (in PNAS January 2, 2001 vol. 98 no. 1 367–372) Bibliografi Author: Sato, Fumihiko ; Hashimoto, Takashi ; Hachiya, Akira ; Tamura, Ken-ichi ; Choi, Kum-Boo ; Morishige, Takashi ; Fujimoto, Hideki ; Yamada, Yasuyuki Topik: berberine; nicotine; polyamine; sanguinarine; scopolamine Bahasa: (EN )     Penerbit: National Academy of Sciences     Tempat Terbit: Washington, D.C.    Tahun Terbit: 2001     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: 011526398v1.pdf (233.62KB; 4 download) Abstract Plant alkaloids, one of the largest groups of natural products, provide many pharmacologically active compounds. Several genes in the biosynthetic pathways for scopolamine, nicotine, and berberine have been cloned, making the metabolic engineering of these alkaloids possible. Expression of two branching-point enzymes was engineered: putrescine N-methyltransferase (PMT) in transgenic plants of Atropa belladonna and Nicotiana sylvestris and (S)-scoulerine 9-Omethyltransferase (SMT) in cultured cells of Coptis japonica and Eschscholzia californica. Overexpression of PMT increased the nicotine content in N. sylvestris, whereas suppression of endogenousPMT activity severely decreased the nicotine content and induced abnormal morphologies. Ectopic expression of SMT caused the accumulation of benzylisoquinoline alkaloids in E. californica. The prospects and limitations of engineering plant alkaloid metabolism are discussed. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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