Detail Chondrocyte Translocation Response to Direct Current Electric Fields   Oleh:  Valhmu, Wilmot B. ; Mauck, Robert L. ; Roy, Rani ; Hung, Clark T. ; Liu, Wendy ; Chao, Pen-Hsiu Grace Jenis: Article from Bulletin/Magazine Dalam koleksi: Journal of Biomechanical Engineering vol. 122 no. 3 (2000), page 261-267. Topik: ELECTRIC FIELD; chondrocyte; translocation; electric fields Ketersediaan Perpustakaan Pusat (Semanggi) Nomor Panggil: JJ52.1 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada     Lihat Detail Induk Isi artikel Using a custom galvanotaxis chamber and time - lapse digital video microscopy, we report the novel observation that cultured chondrocytes exhibit cathodal migration when subjected to applied direct current (DC) electric fields as low as 0.8 V/cm. The response was dose - dependent for field strengths greater than 4 V/cm. Cell migration appeared to be an active process with extension of cytoplasmic processes in the direction of movement. In some cells, field application for greater than an hour induced elongation of initially round cells accompanied by perpendicular alignment of the long axis with respect to the applied field. Antagonists of the inositol phospholipid pathway, U - 73122 and neomycin, were able to inhibit cathodal migration. Cell migration toward the cathode did not require the presence of serum during field application. However, the directed velocity was nearly threefold greater in studies performed with serum. Studies performed at physiologic temperatures (~ 37 °C) revealed a twofold enhancement in migration speed compared to similar studies at room temperature (~ 25 °C). Findings from the present study may help to elucidate basic mechanisms that mediate chondrocyte migration and substrate attachment. Since chondrocyte migration has been implicated in cartilage healing, the ability to direct chondrocyte movement has the potential to impact strategies for addressing cartilage healing / repair and for development of cartilage substitutes. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Kembali

Copyright © 2006, 2007 Unika Atma Jaya, all rights reserved