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ArtikelApplication of Response Surface Method in Optimization of Medium Composition for Xylanase Production by Bacillus halodurans CM1 in Submerged Fermentation  
Oleh: Wibowo, Sara Gustia ; Helianti, Is ; Suryani, Ani ; Wahyuntari, Budiasih
Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Microbiology Indonesia vol. 10 no. 3 (Sep. 2016), page 112-117.
Topik: Bacillus halodurans CM1; production optimization; RSM; xylanase
Fulltext: 501-1253-1-PB_Ros.pdf (692.51KB)
Isi artikelA two level factorial design was performed to optimize xylanase production by alkalothermophilic Bacillus halodurans CM1 using response surface method. The variables involved in this experiment were carbon (X ), 1 nitrogen source (X ) concentration, and pH (X ), corn cob and fish powder were used as carbon and nitrogen 2 3 source respectively. Statistical analysis of the experimental results in the range studied, only carbon source gave significant effect on xylanase production. A second-order model was proposed to represent the enzyme activity as a function of xylan concentration (X ) and pH (X ). The optimum corn cobs concentration was 4.37% (w/v), 1 3 and the fish powder (with high P content) concentration was 1.75% (w/v) and pH 9. These conditions were tested and validated experimentally since the maximum growth rate achieved with these parameters, and the highest xylanase activity observed was 522 U mL-1 after 24 h fermentation.
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