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ArtikelThe Morphological Features of Erythrocytes in Stored Packed Red Cells  
Oleh: Kartini, Dewi Sri ; Muhiddin, Rahmawati ; Arief, Mansyur
Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Clinical Pathology and Medical Laboratory vol. 23 no. 02 (Mar. 2017), page 103-106.
Topik: Storage Time; Crenated Cells; Spherocytes Cells; Bite Cells; Lama Penyimpanan
Fulltext: I01 v23 n2 p103 kelik2017.pdf (539.22KB)
Ketersediaan
  • Perpustakaan FK
    • Nomor Panggil: I01.K
    • Non-tandon: 1 (dapat dipinjam: 0)
    • Tandon: tidak ada
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Isi artikelThe morphology of erythrocyte Packed Red Cells (PRC) is subject to changes during storage at 2o–8oC. In maintaining their viability, erythrocytes require adenosine triphosphate (ATP). The decrease in intracellular ATP level results in damaged membrane lipid, accumulation of intracellular Sodium and Calcium, decreased intracellular potassium and water, cell dehydration, rigidity of membrane and changes in its shape from a disc into crenated red cells, spherocytes and bite cells. This study was aimed to observe the presentation of erythrocyte crenated cells, spherocytes and bite cells of stored PRC at day 3 (control), day 7, day 14 and day 21 from the blood collection date of the blood bag. An observational study with cohort study approach was conducted in August 2015 at the Blood Bank of Dr. Wahidin Sudirohusodo Hospital Makassar. The total sample was 30 blood bag that resulted in 120 slide blood smears. From 30 samples of blood bags comprising blood group A 26.6%, B 13.3%, AB 16.6% and O 43.3%, the increased percentages of crenated cells, spherocytes and bite cells were observed at day 3, day 7, day 14 and day 21. Day 3 of storage was taken as control. Data were processed by Fiedman test and Wilcoxon test with significance level at p<0.001. There was an increase in the percentage of erythrocyte morphology change (crenated cells, spherocytes and bite cells) along with the storage time of packed red blood cells. The use of packed red blood cells was recommended not more than 21 days of storage.
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