Molecular Identification of Phenol-Degrading and Biofilm-Forming Bacteria from Wastewater and Peat Soil  

Oleh:

Khusnuryani, Arifah ; Martani, Erni ; Wibawa, Tri ; Widada, Jaka

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Biotechnology vol. 19 no. 02 (2014), page 99-110.
Topik: bio? lm-forming bacteria; phenol-degrading bacteria; phenotypic and genotypic characterization
Fulltext: 9299-17241_her.pdf (389.08KB)

Isi artikel

Phenol is hazardous aromatic pollutant which needs to be treated to reduce its hazardous effects. Bioremediation using bacteria which can form bio? lm offer an alternative wastewater treatment that is cheaper and environmentally safe. Eighteen strains of phenol-degrading and bio? lm-forming bacteria were isolated from peat soil, also hospital and textile wastewater. Screening for phenol degradation ability of isolates were performed using Folin-ciocalteau reagent, while for bio? lm formation ability were performed using microtiter plate and crystal violet dye. Based on the ability to degrade phenol and to form bio? lm, four isolates (HP3, DOK135, DL120, andATA6) were choosen as phenol-degrading bacteria as well as bio? lm-forming bacteria. Based on phenotypic and genotypic characterization, isolate HP3 was highly similar to Rhodococcus equi strain DSM20307T, while DOK135 was highly similar to Enterobacter mori strain R18-2.The results also suggested that DL120 and ATA6 could be classi? ed to the genus of Micrococcus and Bacillus respectively.

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