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Karakteristik kemiripan genetik Nyamuk Aedes Aegypti di daerah endemis demam berdarah dengue di Kota Palembang Provinsi Sumatera Selatan
Article from Journal - ilmiah nasional - tidak terakreditasi DIKTI
Jurnal Ekologi Kesehatan: Indonesian Journal of health Ecology, The. vol. 10 no. 2 (Jun. 2011)
KEMIRIPAN GENETIK NYAMUK AEDES AEGYPTI.pdf
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One methode to detect early warning done by analyzing the pattern of the array of mosquito vectors. Through the isolation of genomic DNA obtained mosquitoes that are processed through RAPD. Analysis of RAPD products obtained from the results of all the array of DNA amplification is calculated based on the presence or absence of DNA arrays. Analysis of DNA array pattern is intended to obtain the percentage composition of the DNA array patterns by counting the presence of Aedes sp larik pattern. Levels of genetic polymorphisms of Aedes sp mosquitoes as vectors of dengue fever (DHF) by using six kinds of primary (combined) in endemic areas 0.67 (0.6-0.8) and 0.33 monomor phic, while non-endemichas level of 0.4 polymorphic and monomorphic 0,6. DNA array pattern on the Aedes sp in endemic areas is obtained by using the primer combination on average 24.78 (14-33). The highest polymorphism array at Kebon bunga (33 lines), Sukarame, and Lembung Gajah 28 line, Talang dondang 26 line, Skip Ujung, 27 Ilir, and 20 Ilir reach 24 line, and Pahlawan 22 line and non-endemic area Srimulya 9 lines. The percentage of DNA array as an indication of dengue vectors in endemic areas average 57.5% (53.9 to 75.9%) samples from the highest village of Pahlawan (75.9%). From the analysis using the program NTSYS-PC a kinship based on genetic distance matrix value, the highest genetic distance of 1.00 is obtained. In addition, there are three clusters that form the 32.5% level of kinship.
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