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ArtikelDifucosylated oligosaccharide Lewis Y is contained within integrin avß3 on RL95-2 cells and required for endometrial receptivity  
Oleh: Dongmei, Zhang ; Jianxin, Wei ; Jiao, Wang ; Shuai, Liu ; Xiaoqi, Wang ; Qiu, Yan
Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 95 no. 04 (Mar. 2011), page 1446-1451.
Topik: ENDOMETRIAL; LeY; integrin avß3; RL95-2 cells; embryo; endometrium; adhesion
Ketersediaan
  • Perpustakaan FK
    • Nomor Panggil: F02.K.2011.03
    • Non-tandon: 1 (dapat dipinjam: 0)
    • Tandon: tidak ada
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Isi artikelObjective To investigate whether Lewis Y (LeY) carried by integrin avß3 influences integrin avß3–mediated adhesion in an in vitro implantation model. Design Laboratory research. Setting Reproduction and glycobiology research laboratory. Intervention(s) Specific antibody blockage of LeY or integrin avß3 and knockdown of FUT4 expression in RL95-2 cells by transient transfection of FUT4 siRNA. Main Outcome Measure(s) The expression of integrin avß3 and LeY in both endometrial tissues and RL95-2 cells was measured. LeY carried by integrin avß3 was identified by examining the immunoprecipitated integrin avß3. The effect of knocking down FUT4 on the expression of integrin avß3 and LeY and their impact on the adhesion of JAR cells to the RL95-2 cells were assessed. Result(s) Integrin avß3 and LeY are expressed in both secretory-stage human endometrial tissue and in RL95-2 cells. Although integrin avß3 carries LeY, knocking down FUT4 expression only reduces the expression of LeY but not of integrin avß3. Knocking down FUT4, antibody blockade of LeY or integrin avß3 consistently decreases the adhesion of JAR cells to the RL95-2 cells and prevents focal adhesion kinase (FAK) phosphorylation. Conclusion(s) LeY carried by integrin avß3 plays a critical role on the attachment of JAR cells to the RL95-2 cells and activates integrin avß3/FAK signaling.
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