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Primate model of metaphase I oocyte in vitro maturation and the effects of a novel glutathione donor on maturation, fertilization, and blastocyst development
Ryan, John P.
Saunder, Douglas M.
Article from Journal - ilmiah internasional
Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 95 no. 04 (Mar. 2011)
Glutathione ethyl ester
oocyte in vitro maturation
male pronucleus formation
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Lihat Detail Induk
Objective To assess the effect of glutathione ethyl ester (GSH-OEt) on the development of macaque metaphase (MI) oocytes as a model for human MI oocyte in vitro maturation (IVM). Design Prospective cohort study. Setting Nonhuman primate assisted reproductive technology program. Animal(s) Twenty-three Macaca fascicularis females aged 6.5–12.5 years. Intervention(s) Ovarian stimulation and maturation of MI oocytes in  human tubal fluid (HTF),  mCMRL-1066,  mCMRL-1066+GSH-OEt 3 mM, or  mCMRL-1066+GSH-OEt 5 mM. Oocytes were assessed for maturation after 4–6 hours (early) and 18–20 hours (late) of culture. Mature oocytes were inseminated or subjected to glutathione (GSH) assay. Zygotes were cultured to the blastocyst stage for total differential cell counts. Main Outcome Measure(s) Oocyte maturation rate, GSH content, pronuclear formation and blastocyst development, and cell number were compared between IVM treatment groups and sibling in vivo matured (IVO) MII oocytes. Result(s) Compared with HTF, mCMRL-1066 supported higher rates of normal fertilization and blastocyst development in early but not late maturing MI-MII oocytes. Five micromoles of GSH-OEt significantly increased blastocyst total cell and inner cell mass cell number in early MI-MII oocytes compared with IVO and IVM controls. GSH-OEt significantly increased oocyte GSH content and fertilization in late maturing oocytes but not blastocyst development. Conclusion(s) GSH-OEt positively affects the development of early and late maturing IVM oocytes.
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