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A Slicer-Mediated Mechanism for Repeat-Associated siRNA 5' End Formation in Drosophila
Gunawardane, Lalith S.
Nishida, Kazumichi M.
Siomit, Mikiko C.
Article from Bulletin/Magazine
SCIENCE (keterangan: ada di Proquest) vol. 315 no. 5818 (Mar. 2007)
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In Drosophila, repeat-associated small interfering RNAs (rasiRNAs) are produced in the germ line by a Dicer-independent pathway and function through the PIWI subfamily of Argonautes to ensure silencing of retrotransposons. We sequenced small RNAs associated with the PIWI subfamily member AG03. Although other members of PIWI, Aubergine (Aub) and Piwi, associated with. rasiRNAs derived mainly from the antisense strand of retrotransposons, AG03-associated rasiRNAs arose'mainly from the sense strand. Aub- and Piwi-associated rasiRNAs showed a strong preference for uracil at their S' ends, and AG03-associated rasiRNAs showed a strong preference for adenine at nucleotide 10. Comparisons between AG03- and Aub-associated rasiRNAs revealed pairs of rasiRNAs showing complementarities in their first 10 nucleotides. Aub and AG03 exhibited Slicer activity in vitro. These data support a model in which formation of a S' terminus within rasiRNA precursors is guided by rasiRNAs originating from transcripts of the other strand in concert with the Slicer activity of PIWI.
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