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ArtikelA Slicer-Mediated Mechanism for Repeat-Associated siRNA 5' End Formation in Drosophila  
Oleh: Gunawardane, Lalith S. ; Saito, Kuniaki ; Nishida, Kazumichi M. ; Miyoshi, Keita ; Kawamura, Yoshinori ; Nagami, Tomoko ; Siomi, Haruhiko ; Siomit, Mikiko C.
Jenis: Article from Bulletin/Magazine
Dalam koleksi: SCIENCE (keterangan: ada di Proquest) vol. 315 no. 5818 (Mar. 2007), page 1587.
Topik: Molecular Biology
Ketersediaan
  • Perpustakaan FK
    • Nomor Panggil: S01.K.2007.03
    • Non-tandon: 1 (dapat dipinjam: 0)
    • Tandon: tidak ada
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Isi artikelIn Drosophila, repeat-associated small interfering RNAs (rasiRNAs) are produced in the germ line by a Dicer-independent pathway and function through the PIWI subfamily of Argonautes to ensure silencing of retrotransposons. We sequenced small RNAs associated with the PIWI subfamily member AG03. Although other members of PIWI, Aubergine (Aub) and Piwi, associated with. rasiRNAs derived mainly from the antisense strand of retrotransposons, AG03-associated rasiRNAs arose'mainly from the sense strand. Aub- and Piwi-associated rasiRNAs showed a strong preference for uracil at their S' ends, and AG03-associated rasiRNAs showed a strong preference for adenine at nucleotide 10. Comparisons between AG03- and Aub-associated rasiRNAs revealed pairs of rasiRNAs showing complementarities in their first 10 nucleotides. Aub and AG03 exhibited Slicer activity in vitro. These data support a model in which formation of a S' terminus within rasiRNA precursors is guided by rasiRNAs originating from transcripts of the other strand in concert with the Slicer activity of PIWI.
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