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Cold - Shock Induced High - Yield Protein Production in Escherichia Coli
Swapna, G. V. T.
Mal, Tapas K.
Takayama, Masanori Mitta
Montelione, Gaetano T.
Article from Journal - ilmiah internasional
Nature Biotechnology: The Science and Business of Biotechnology vol. 22 no. 7 (Jul. 2004)
cold shock induced
high yield protein production
Perpustakaan Pusat (Semanggi)
1 (dapat dipinjam: 0)
Lihat Detail Induk
Overexpression of proteis in escherichia coli at low temperature improves their solubility and stability. Here we apply the unique features of the cspA gene to develop a series of expression vectors, termed pCold vectos, that drive the high expression of cloned genes upon induction by cold shock. Several proteins were produced with very high yields, including E. Coli EnvZ ATP binding domain (EnvZ-B) and Xenopus laevis calmodulim (CaM). The pCold vector system can also be used to selectively enrich target proteins with isotopes to study their properties in cell lysates using NMR spectroscopy. We have cloned 38 genes from a range of prokaryotic and eurokaryotic organisms into both pCold and pET14 (ref. 3 ) systems and found that pCold vectors are highly complementary to the widely used pET vectors.
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