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An Engineered Epigenetic Transgene Switch in Mammalian Cells
Kramer, Beat P.
Viretta, Alessandro Usseglio
Baba, Marie Daoud-El
Article from Journal - ilmiah internasional
Nature Biotechnology: The Science and Business of Biotechnology vol. 22 no. 7 (Jul. 2004)
engineered epigenetic transgene switch
Perpustakaan Pusat (Semanggi)
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Lihat Detail Induk
In multicellular system cell identity is imprinted by epigenetic regulation circuits, which determine the global transcriptome of adult cells in a cell phenotype specific manner. By combining two repressors, which control each other's expression, we have developed a mammalian epigenetic circuitry able to switch between two stable transgene expression states after transient administration of two alternate drugs. Engineered chinese hamster ovary cells (CHO-L1) showed toggle switch specific expression profiles of a human glycoprotein in culture, as well as after microencapsulation and implantation into mice. Switch dynamics and expression stability could be predicted with mathematical models. Epigenetic transgene control through toggle switches is an important tool for engineering artificial gene networks in mammalian cells.
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