Anda belum login :: 07 Jul 2022 05:58 WIB
Single - Chain Antibodies Against a Plant Viral RNA Dependent RNA Polymerase Confer Virus Resistance
Nagy, Peter D.
Article from Journal - ilmiah internasional
Nature Biotechnology: The Science and Business of Biotechnology vol. 22 no. 7 (Jul. 2004)
single chain antibodies
plant viral RNA
Perpustakaan Pusat (Semanggi)
1 (dapat dipinjam: 0)
Lihat Detail Induk
Crop loss due to viral diseases is still a major problem for agriculture today. We present a strategy to achieve virus resistance based on the expression of single chain Fv fragments (scFvs) against a conserved domain in a plant viral RNA - dependent RMNA polymerase (RdRp), a key enzyme in virus replication. The selected scFvs inhibited complementary RNA synthesis of different plant virus RdRps in vitro and virus replication in planta. Moreover, the scFvs also bound to the RdRp of the distantly related hepatitis C virus. T1 and T2 progeny of transgenic lines of Nicotiana benthamiana expressing different scFvs either in the cytosol or in the endoplasmic reticulum showed varying degrees of resistance against four plant viruses from different genera, three of which belong to the tombusviridae family. Virus resistance based on antibodies to RdRps adds another tool to the repertoire for combating plant viruses.
Klik untuk menuliskan opini Anda tentang koleksi ini!
Copyright © 2006, 2007
Unika Atma Jaya
, all rights reserved
Process time: 0.015625 second(s)