Inhibitory Activity of Lactobacillus plantarum U10 Isolated from Tempoyak (Fermented Durian) Made in Indonesia against Salmonella typhi  

Oleh:

Sogandi ; Mustopa, Apon Zaenal ; Artika, I Made ; Budiarto, Bugi Ratno

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Microbiology Indonesia vol. 9 no. 2 (Jun. 2015), page 73-81.
Topik: bacteriocin; L. plantarum U10; mode of action; purification
Fulltext: 323-935_her.pdf (1,007.51KB)

Isi artikel

Lactobacillus plantarum U10 produced bacteriocin was isolated from a traditionally fermented food “tempoyak” from Sumatera Island in Indonesia. Production of the bacteriocins started at early exponential phase and reached maximum level at early stationary phase. Furthermore, plantaricins U10 was purified by ammonium sulphate precipitation followed by gel filtration chromatography. L. plantarum U10 produced two bacteriocins with a molecular mass of approximately 4.5 and 9.8 kDa by SDS-PAGE. The mode of action of plantaricins U10 was identified as bactericidal agents against Salmonella typhi ATCC25241 as proven by CFU counting and SEM micrographs that showed differences in cell structures between treated cells and the non-treated control. SEM examination also confirmed structural destruction of membrane cells integrity and considerable morphological alteration of S.typhi.

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