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Vitrification of mouse embryos with super-cooled air
Larman, Mark G.
Article from Journal - ilmiah internasional
Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 95 no. 04 (Mar. 2011)
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Objective To develop a closed vitrification device (i.e., one that requires no direct contact with liquid nitrogen) for successful cryostorage of embryos. Design Prospective laboratory research study. Setting University-based research laboratory. Animal(s) F1 mice and mouse embryos. Intervention(s) Mouse embryos were vitrified using two methods and compared with nonvitrified controls. Embryos were vitrified on a device by either  presealing it within a straw before plunging into liquid nitrogen or  placing the straw into liquid nitrogen so that the air inside the straw is super-cooled before inserting the device holding the embryos. Main Outcome Measure(s) Survival, subsequent embryo development, and cell number were determined. Embryos were also cryopreserved for 12 months to assess long-term storage. Synchronized ETs were performed to compare viability with nonvitrified control embryos. Result(s) All embryos survived with both techniques. Day-4 and -5 embryo development was comparable between the two vitrification methods. Use of the presealing method resulted in a significantly lower mean cell number than the postsealing method and control. Long-term storage did not affect subsequent embryo development or cell number. The implantation and fetal development rates of embryos vitrified with super-cooled air were comparable to those for nonvitrified control embryos. Conclusion(s) These data demonstrate that a closed vitrification device (Rapid-i), which does not require direct liquid nitrogen contact for vitrification, is appropriate for vitrification and long-term storage of mouse embryos.
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