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Detection of Mycobacterium avium subspecies paratuberculosis in formula milk from Bogor using PCR IS 900
Nugroho, Widagdo Sri
Lukman, Denny Widaya
Hassan, Abdulwahed Ahmed
Article from Journal - ilmiah nasional - terakreditasi DIKTI
Medical Journal of Indonesia vol. 17 no. 03 (Jul. 2008)
growing up formula milk.
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Crohn's disease (CD) that becomes a public health concern in develoved countries shows similarities in clinical sign and pathological features with Johne's disease (JD) in ruminants infected by Mycobacterium avium subspecies paratuberculosis (MAP). Few researches conducted in Europe, the USA, and Australiashowed relationship between MAP, CD, JD and dairy cows that were seropositive for MAP , and this could be a serius problem id diary farm animals and human health in the future . The aim of this study was to detect MAP inthe growing up formula milk. Fifty sample from five established factories ware taken from supermakets in Bogor. Polymerrase chain reaction method (PCR) with insertion sequence (IS) 900 as primer and culture in Herrold's egg yolk media with mycobatin J (HEYM J) as agold standard were used in this study. Neither MAP grew up in HEYM J medium after 20 weeks of culture periode nor positive sample by PCR IS 900 were found. Although there were no positive sample found in this study, futher extensive and comprehensive studies on MAP should be done with more and varied sample, as well as in human to provide data on MAP in Indonesia.
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