Aktivitas Antioksidan dan Toksisitas Senyawa Bioaktif dari Ekstrak Rumput Laut Hijau Ulva reticulata Forsskal  

Oleh:

Swasono R. Tamat ; Wikanta, Thamrin ; Maulina, Lina S.

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Jurnal Ilmu Kefarmasian Indonesia vol. 05 no. 01 (Apr. 2007), page 31 - 36.
Topik: antioxidant; toxicity; BSLT; DPPH; Ulva reticulata Forsskal

Ketersediaan

Perpustakaan FK Nomor Panggil: J27.K.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Research on the antioxidative activity and toxicity of the bioactive substance of the green seaweed Ulva reticulata Forsskal extract had been carried out. the bioactive substance of the green seaweed Ulva reticulata Forsskal was extracted using methanol. The phytochemistry test on the methanol extract showed that the extract contained triterpenoid compound. The methanol extact was then partitioned using n-hexane-water (1:1) and chloroform-water (1:1). Each of the extract was then dried using freeze dryer. The antooxidative activity test of each fraction against free radical 1,1-diohenyl-2-picrilhydrazil (DPPH) showed that the water extracty has the highest activity with IC50 of 365,95... . The toxicity test of each fraction against brine shrimp Artemia salina (Brine Shrimp Lethaly Test, BSLT) showed that the chloroform extract showed the highest activity with LC50 of 250,67... . The water extract was then fractionated through silica column chromatography. The activity test of each fraction DPPH and BSLT showed that fraction 1 has the highest activity with LC50 of 100... and IC50 of 270,31... . Identification using gas chromatography - mass spectrometric method showed that the antioxidative substance presence in the active fraction was probably nonyl phenol (C5H24O).

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