Produksi dan Penetapan Kadar ß-glukan dari Tiga Galur Saccharomyces cerevisiae dalam Media Mengandung Molase  

Oleh:

Kusmiati ; Nuswantara, S. ; Isnaini, Nita

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Jurnal Ilmu Kefarmasian Indonesia vol. 05 no. 01 (Apr. 2007), page 7 - 16.
Topik: beta-glucan; Saccharomyces cerevisiae; RTA; RN4; and SC strains; molase

Ketersediaan

Perpustakaan FK Nomor Panggil: J27.K.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Beta-glucan extracted from the cell membrane of Saccharomyces cerevisiae has been used as food and medical ingredients.Molase as a waste product of the cane-sugarindustry, containing4-9% of glucose and other nutrients, has been investigated to subtitute glucose in the YPG fermentation media of Saccharomyces cerevisiae, and ro improve the yield of beta-glucan by three strains of Saccharomyces cerevisiae (RTA, RN4, and SC). Beta glucan and protein concentration were determined by spectrophotometric method at ... 490 nm and 750 nm respectively. The result showed that molase can be used as glucose-substitute in the YPG media for the production of beta glucan by three strains of Saccharomyces cerevisiae. The best yield for each strain was as follows : RTA strain in a media containing molase (8% v/v glucose equivalent) produced 61, 79% w/w of beta glucan;RN4 strain in a media containing molase (1% v/v glucose equivalent) produced 98,42% w/w of beta glucan; SC strain in a media containing molase (2% v/v glucose equivalent) produce 56,48% w/w of beta glucan. One way Anova followed by the Tukey Bonferroni test indicated that molase can be used as substitute of glucose source in the YPG fermentation media, and significantly increased the beta-glucan yield by all the three strains of Saccharomyces cerevisiae, as well as reducing the proteins contents. The highest beta-glucan yield (98,42% w/w) was attained by the RN4 strain in a media containing molase (1% v/v glucose equivalent) with a protein impurity of only 10,53% w/w, while the RN4 and RTA strains produced a higher beta-glucan yield than that of the SC strain.

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