Direct Cloning of a Xylanase Gene from Pawan-Riau Hot Spring  

Oleh:

Helianti, Is

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Hayati Journal of Biosciences vol. 14 no. 2 (Jun. 2007), page 54-58.
Topik: B-1; 4-Endoxylanase; Metagenomic DNA; Pawan-Riau Hot-spring
Fulltext: 105.pdf (134.34KB)

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: HH13.9 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

A functional gene containing an Open Reading Frame (ORF) encoding a B-1, 4-endoxylanase glycosyl hydrolase family 11 was cloned directly using metagenomic PCR-cloning method from Pawan Hot Spring sample in Riau. The gene consisted of 642 nucleotides, encoded for, 213 amino acids. The amino acid sequence analysis using BLAST showed that the gene has high homology (93%) with xylanase gene from Bacillus subtiis. The gene showed its function when it was subcloned into an expression vector and overexpressed in E. coil. The crude extract of the recombinant enzyme had activity for 170 U/mI at 50 °C. The result of this work showed that metagenomic approach was a powerful short cut method to obtain recombinant biocatalyst that was useful for industrial application.

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