Invariant Natural Killer T Cells in Asthma and Chronic Obstructive Pulmonary Disease  

Oleh:

Vijayanand, Pandurangan ; Seumois, Gregory ; Pickard, Chris ; Powell, Robert M. ; Angco, Gilbert ; Sammut, David ; Gadola, Stephan D. ; Friedmann, Peter S. ; Djukanovic, Ratko

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: The New England Journal of Medicine (keterangan: ada di Proquest) vol. 356 no. 14 (Apr. 2007), page 1410.

Ketersediaan

Perpustakaan FK Nomor Panggil: N08.K.2007.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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BACKGROUND The number of type 2 helper CD4+ T cells is increased in the airways of persons with asthma. Whether the majority of these cells are class II major-histocompatibilitycomplex-restricted cells or are among the recently identified CDld-restricted invariant natural killer T cells is a matter of controversy. We studied the frequency ofinvariant natural killer T cells in the airways of subjects with mild or moderately severe asthma to investigate the possibility of an association between the number of invariant natural killer T cells in the airway and disease severity. We also studied whether an increased number of these cells is a feature of chronic obstructive pulmonary disease (COPD). METHODS We enumerated invariant natural killer T cells by flow cytometry with the use of CDld tetramers loaded with a-galactosylceramide and antibodies specific to the invariant natural killer T-cell receptor in samples ofbronchoalveolar-Iavage fluid, induced sputum, and bronchial-biopsy specimens obtained from subjects with mild or moderately severe asthma, subjects with COPD, and healthy control subjects. Real-time polymerase-chain-reaction analysis was performed on bronchoalveolar-Iavage cells for evidence of gene expression of the invariant natural killer T-cell receptor. RESULTS Fewer than 2% of the T cells obtained from all subjects on airway biopsy, bronchoalveolar lavage, and sputum induction were invariant natural killer T cells, with no significant differences among the three groups of subjects. No expression of messenger RNA for the invariant natural killer T-cell-receptor domains Va24 and VJ311 was detected in bronchoalveolar-Iavage cells from subjects with asthma. CONCLUSIONS Invariant natural killer T cells are found in low numbers in the airways of subjects with asthma, subjects with COPD, and controls.

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