Mutation and Characterization of an Albino Mutant of Monascus sp. Isolated from the Cikapundung River, Bandung  

Oleh:

Milanda, Tiana ; Dhanutirto, Haryanto ; Gusdinar, Tutus ; Wibowo, Marlia Singgih

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Microbiology Indonesia vol. 01 no. 01 (Apr. 2007), page 19-22.
Topik: Monascus sp.; Ethyl Methanesulfonate; Random Amplification Polymorphic DNA; Albino Mutant; Monascidin
Fulltext: Tiana_Marlia_Tutus_Haryanto.pdf (634.65KB)

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: MM78.1 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada	Lihat Detail Induk
Perpustakaan FK Nomor Panggil: M53.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada	Lihat Detail Induk

Isi artikel

Monascus sp. isolated from Cikapundung River, Bandung was mutated using ethyl methanesulfonate (2.5%, 90 mm). Previously, this wild type was identified as Monascus purpureus ITBCC-HD-F0O1 employing random amplification polymorphic DNA (RAPD). Stability of the mutant was observed using color consistency and mutant stability (sub-culturing for five generations) tests. Genetic variation of the mutant (M. purpureus ITBCC-HDF002) was confirmed by RAPD. One of the DNA bands of 1150 bp was found in the albino mutant but not in the wild type, so it was considered as a genetic variation resulting from the mutation process. The albino mutant was characterized by comparing tlte growth curve, biomass production curve, and the monascidin A production curve of both strains i.e. wild type and the albino mutant. Monascidin A production of the mutant was higher than that of the wild type.

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