Detail Probing Transcription Factor Dynamics at the Single-Molecule level in a Living Cell   Oleh:  Elf, ]ohan ; Li, Gene-Wei ; Xielt, X. Sunney Jenis: Article from Bulletin/Magazine Dalam koleksi: SCIENCE (keterangan: ada di Proquest) vol. 316 no. 5828 (May 2007), page 1191. Ketersediaan Perpustakaan FK Nomor Panggil: S01.K.2007.05 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada     Lihat Detail Induk Isi artikel Transcription factors regulate gene expression through their binding to DNA. In a living Escherichia coli cell, we directly observed specific binding of a lac repressor, labeled with a fluorescent protein, to a chromosomal lac operator. Using single-molecule detection techniques, we measured the kinetics of binding and dissociation of the repressor in response to metabolic signals. Furthermore, we characterized the nonspecific binding to DNA, one-dimensional (lD) diffusion along DNA segments, and 3D translocation among segments through cytoplasm at the single-molecule level. In searching for the operator, a lac repressor spends -90% of time nonspecifically bound to and I diffusing along DNA with a residence time of <5 milliseconds. The methods and findings can be generalized to other nucleic acid binding proteins. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Kembali

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