EKSPRESI CYTOSOLIC ASPARTATE-SPECIFIC CYSTEINE PRO TEASE-3 (CASPASE.3) PADA JARINGAN HATI RATTUS NORVEGICUS (WISTAR) SETELAH PEMBERIAN SUBKRONIK AFLATOKSIN BI (AFB1). EXPRESSION OF CYTOSOLIC ASPARTATE-SPECIFIC CYSTEINE PROTEASE-3 (CASPASE-3) IN THE LIVER TISSUE OF RATTUS NORVEGICUS (WISTAR) FOLLOWING SUBCHRONIC ADMINISTRATION OF AFLATOXIN-BI (AFB1)  

Oleh:

Maritha, Indah Dina ; Supranowo ; Lyrawati, Diana

Jenis: Article from Journal - ilmiah nasional
Dalam koleksi: Jurnal Kedokteran Brawijaya vol. 22 no. 03 (Dec. 2006), page 107-112.
Topik: Aflatoxin BI; Caspase-3; Rattus Noivegicus; Liver

Ketersediaan

Perpustakaan FK Nomor Panggil: J34.K.2004-2006.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Aflatoksin B1 (AFB1) is one of the toxic agents produced byAspergillus flavus that frequently contaminates foods not properly stored. AFBI undergoes biotransformation which may result in the production of reactive oxygen species (ROS) hazardous to liver cells. Following a cascade of ox/dative reaction, ROS will cause the mitochondria to release cytochrome c which subsequently activates caspase-3, leading to apoptosis. In this present study we evaluated the effect of aflatoxin BI on the expression of caspase-3 in the liver AFBI was administered per oral, at different dosage and length of exposure, subchronically. This study was carried out as a factorial designed experiment with two factors. The first was dosage factor i.e 0, 10, 15 and 20 pg (0; 0,05; 0,075; 0,1 IgIg BW) and the second was exposure time factori.e. 12, 16 and 20 weeks. Rattus norvegicus strain Wistar aged approximately eight weeks old and weighed 180-200 g were used as the experimental animals. The expression of caspase-3 was examined by using immunohistochemistry The results showed that the expression of caspase-3 increased significantly (p = 0,000) with the escala¬tion of AFB1in dosage and/or exposure time (p = 0,001). In the interaction between dose and exposure time of AFB1 an increase in the expression of caspase-3 was also obseived (p = 0,000). Interestingly, these studies also revealed that in the liver tissues there was a limitation in the expression of caspase-3, where the raising of further AFBl dosage and length of exposure were not followed by further increase of the caspase-3. The caspase-3 expression tends to decrease after the administration of AFBI 20 ug for 16 weeks. Microscopic obse,vations demonstrated that the number of the liver cells was declining with the increasing dosage and length of exposure to AFBI. Combining those data, it can be concluded that reduction of caspase-3 following certain high dosage and length of exposure to AFBI maybe attributed to the loss of the majority of the liver cells due to apoptosis, thus only a small amount of the remaining liver cells could response and express caspase-3.

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali