PENGARUH PEMBERIAN EKSTRAK BIJI JINTEN HITAM (Nigella sativa) TERHADAP KADAR GSH, MDA, JUMLAH SERTA FUNGSI SEL MAKROFAG ALVEOLAR PARU TIKUS WISTAR YANG DIPAPAR ASAP ROKOK KRONIS. THE EFFECT OF BLACK SEED CRUDE EXTRACT (Nigella sativa) ON GSH AND MDA LEVEL, NUMBER AND FUNCTION OF LUNG ALVEOLAR MACROPHAGE OF WISTAR RAT WHICH EXPOSED BY CHRONIC CIGARETTE SMOKE  

Oleh:

Marwan ; Widjajanto, Edi ; Karyono, Setyawati

Jenis: Article from Journal - ilmiah nasional
Dalam koleksi: Jurnal Kedokteran Brawijaya vol. 21 no. 03 +suplemen (Dec. 2005), page 111-120.
Topik: Black Seed Crude Extract; GSH; MDA; Reactive Oxygen Intermediate; Phagocytosis
Fulltext: 111-120 - Bernard.pdf (371.36KB)

Ketersediaan

Perpustakaan FK Nomor Panggil: J34.K.2004-2006.01 Non-tandon: 2 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Cigarette smoke is the main risk factor of chronic obstructive pulmonary disease (COPD). Several studies about COPD has focused on the oxidative stress in lung. Oxidative stress was related to inactivation of antiprotease enzymes, aitway epithella! destruction and proinflammatory genes expression. Antioxidant may useful for treatment of COPD. Black seed (Nigela sativa) studies have showed that black seed has antioxidant activity. The aim of this research was to prove that black seed crude extract can protect wistar rat lung from oxidative stress which was caused by chronic cigarette smoke exposure. This experimental research used randomized complete design with control group. Thirty wistar strain of Rattus novergicus, 2.5 to 3 months old, 150 — 250 grams of weight were divided into five groups. First group was used as negative control group (without any treatment), second group as positive control group (only cigarette smoke exposure treatment), third group as cigarette smoke exposure and black seed crude extract dose 0.6 grfkilogram of body weight’day (group A), cigarette smoke exposure and black seed crude extract dose 1.2 grikilogram of body weight/day (group B) and cigarette smoke exposure and black seed crude extract dose 2.4 grlkilogram of body weight/day (group C). After 3 months five parameters were measured in this research. They were GSH and MDA of/eve/lung tissue, number and phagocytosis function also reactive oxygen intermediate (ROl) secretion of alveolar macrophage. All data from five parameters in this study were analyzed by the Tukey HSD test and one way anova. MDA level of group C was 0.40900 ±0.256992 nmollgr. wet tissue, significantly lower than positive control group. GSH level of group C was 1.31450 ± 0.035707 pmoL/gr. wet tissue, not significantly higher than positive control group. The number of alveolar macrophage from group C significantly lower than positive control group, phagocytosis function and RO! secretion function from alveolar macrophage were significantly lower than the positive control group (p<0.05). The crude extract of black seed can protect wistar rat lung from oxidative stress which is caused by chronic cigarette smoke exposure.

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