Perbandingan Tan Thiam Hok, Ziehl Neelsen dan Fluorokrom Sebagai Metode Pewarnaan Basil Tahan Asam untuk Pemeriksaan Mikroskopik Sputum  

Oleh:

Risdiayani, E. ; Nilawati, S. ; Prawoto ; Rosana, Yeva ; Alisyahbana, B ; Parwati, I. ; Melia, Wia ; Sudiro, T. Mirawati ; Karuniawati, A.

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Makara Seri Kesehatan vol. 9 no. 1 (2005), page 29-33.
Topik: Acid Fast Staining; Sputum; Pulmonary Tuberculosis; Tan Thiam Hok; Ziehl Neelsen; Fluorochrome; Lowenstein Jensen.

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: MM75.1 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Comparison of Tan Thiam Hok, Ziehi Neelsen and Fluorochrome as Acid-Fast Bacilli Staining Methods in Sputum. Because of its simplicity, rapidity, low cost and relatively sensitive, sputum acid-fast bacilli (AFB) smear microscopy is the primary tool for detecting pulmonary tuberculosis and follow up of therapy. This experiment is aimed to determine the best acid-fast staining method that can be used for i~otitine laboratory examination, especially in the high burdened clinical laboratory. We compared the sensitivity, specivity, and the positive and negative predictive value of 3 kinds of methods : Tan Thiam Hok, Ziehi Neelsen and Fluorochrome, using culture on Lowenstein-Jensen media as the gold standard. The smear results were observed using IUTLD scale. Twenty seven of 98 sputwn specimens from 98 patients with clinical suspicion of tuberculosis (27,6 %) were positive by culture. The sensitivity of Tan Thiam Hok, Ziehi Neelsen and Fluorochrome were 62,9%, 8 1,5% and 92,6%, while the specivity were 92,9%, 9 1,6% and 9 1,1% respectively. The positive predictive value were 77,3 %, 78,6 %, 71,4 %, and the negative predictive value were 86,8 %, 92,9 %, 96,8 % respectively. Although fluorochrome gave the highest sensitivity, it needs special expensive equipments. We conclude that Ziehi Neelsen is still the method of choice for detecting AFB in sputum microscopically.

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