Excentral cleavage of ß-carotene in vivo in a healthy man  

Oleh:

Ho, Charlene C ; Moura, Fabiana F de ; Kim, Seung-Hyun ; Clifford, Andrew J

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: The American Journal of Clinical Nutrition vol. 85 no. 03 (Mar. 2007), page 770.
Topik: ß-carotene • ß-apo-carotenal • humans • vitamin A • 14C

Ketersediaan

Perpustakaan FK Nomor Panggil: A07.K.2007.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

1 From the Department of Nutrition, University of California, Davis, Davis, CA. Background:Excentral cleavage of ß-carotene to retinoids and apocarotenoids occurs in vitro and in animal models. Whether it occurs in humans is unclear. Objective:We tested the hypothesis of whether humans can cleave ß-carotene excentrally. Design:A healthy man was given an oral dose of all-trans [10,10',11,11'-14C]-ß-carotene (1.01 nmol; 100 nCi). Its fate and that of its metabolites were measured in serial plasma samples. Its fate in feces and urine was also measured over time. Selected plasma samples were spiked with reference standards of retinol, ß-apo-12'-carotenal, ß-apo-8'-carotenal, 13-cis-retinoic acid, all-trans-retinoic acid, ß-carotene-5,6-epoxide, all-trans-ß-carotene, and retinyl palmitate and subjected to reverse-phase HPLC fractionation. The plasma, plasma fractions, urine, and feces were measured for 14C with the use of accelerator mass spectrometry. Results: Sixty-five percent of administered 14C was absorbed, and 15.7% was eliminated in urine during the first 21 d after dosing. 14C-ß-carotene and 14C-retinyl palmitate appeared in plasma 0.25 d after the dose. 14C-ß-carotene and 14C-retinol both appeared at 0.5 d only. On day 3 after the dose, 2 large 14C peaks appeared in plasma: one matched the retention time of ß-apo-8'-carotenal, and the other did not match any of the reference standards used. The delayed appearance of 14C-ß-apo-8'-carotenal in plasma suggests that the excentral cleavage occurred after the 14C-ß-apo-8'-carotene was absorbed into the body.

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