Begomovirus Associated with pepper Yellow Leaf Curl Disease in West Java, Indonesia  

Oleh:

Hidayat, Sri Hendrastuti ; Chatchawankanpanich, Orawan ; Rusli, Eliza ; Aidawati, Noor

Jenis: Article from Journal - ilmiah nasional
Dalam koleksi: Jurnal Mikrobiologi Indonesia vol. 11 no. 2 (Sep. 2006), page 87-90.
Topik: DISEASE; begomovirus; common region; leaf curl disease

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: JJ139 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Chilli pepper (capsicum frutescens) showing yellow leaf curl from west java were analysed for the presence of a geminivirus. Detection using specific primers for geminivirus was conducted followung whitefly (bemisia tobaci genn) transmission. A DNA fragment of 1,6 kb was amplified in the polymerase chain reaction using primers which anneal within the replication initiator protein gene and coat protein gene. DNA clone of the polymerase chain reaction product was then sequenced. The stem loop region was found, included the conserved nonanucleotide sequence TAATATTAC present in all gminivirus. The geninivirus associated with yellow leaf curl disease in pepper showed the highest sequence identity 93 and 98% with pepper yellow leaf curl indonesia virus from C. annuum and pepper yellow leaf curl indonesia virus from lycopersicon esculentum, respectively.

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