Characterization and Identification of Vaccine Candidate Proteins Through Analysis of The Group a Streptococcus Surface Proteome  

Oleh:

Rodriguez-Ortega, Manuel J. ; Norais, Nathalie ; Bensi, Giuliano ; Liberatori, Sabrina ; Capo, Sabrina ; Mora, Marirosa ; Scarselli, Maria ; Doro, Francesco ; Ferrari, Germano ; Garaguso, Ignazio ; Maggi, Tiziana ; Neumann, Anita ; Covre, Alessia ; Telford, John L. ; Grandi, Guido

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 24 no. 2 (Feb. 2006), page 191-197.
Topik: VACCINE; vaccine; protein; proteome

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.4 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

We describe a proteomic approach for identifying abcterial surface exposed proteins quickly and reliably for their use as vaccine candidates. Whose cells are treated with proteases to selectively digest protruding proteins that are subsequently identified by mass spectrometry analysis of the released peptides. When applied to the sequenced M1_SF370 group a streptococcus strain 68 PSORT predicted surface associated proteins were identified, including most of the protective antigens described in the literature. The number of surface exposed proteins varied from strain to strain, most likely as a consequence of different capsule content. The number of surface exposed proteins varied from strain to strain, most likely as a consequence of different capsule content. The surface exposed proteins of the highly virulent M23_DSM2071 strain included 17 proteins, 15 in common with M1_SF370. When 14 of the 17 proteins were expressed in E. Coli and tested in the mouse for their capacity to confer protection against a lethal dose of M23_DSM2071, one new protective antigen (Spy0416) was identified. This strategy overcomes the difficulties so far encountered in surface protein characterization and has great potential in vaccine discovery.

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