Quantitative Mouse Brain Proteomics Using Culture Derived Isotope Tags as Internal Standards  

Oleh:

Ishihama, Yasushi ; Sato, Toshitaka ; Tabata, Tsuyoshi ; Miyamoto, Norimasa ; Sagane, Koji ; Nagasu, Takeshi ; Oda, Yoshiya

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 23 no. 5 (Mei 2005), page 617-621.
Topik: proteomics; brain proteomics; isotope tags

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.3 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

An important challenge for proteomics is to be able to compare absolute protein levels across biological samples. Here we introduce an approach based on the use of culture derived isotope tags (CDITs) for quantitative tissue proteome analysis. We cultured Neuro 2A cells in a stable isotope enriched medium and mexed them with mouse brain samples to serve as internal standards. Using CDITs, we identified and quantified a total of 1,000 proteins, 97 -98% of which were expressed in both mouse whole brain and neuro2A cells. ADITs also allow comprehensive and absolute protein quantification. Synthetic unlabeled peptides were used to quantify the corresponding proteins labeled with stable isotopes in neuro2A cells, and the results were used to obtain the absolute amounts of 103 proteins in mouse whole brain. The expression levels correlated well with those in neuro2A cells. Thus, the use of CDITs allows both relative and absolute quantitative proteome studies.

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali