In Vivo Detection of Amyloid - B Deposits by Near - Infrared Imaging Using an Oxazine - Deriavtive Probe  

Oleh:

Hintersteiner, Martin ; Enz, Albert ; Frey, Peter ; Jaton, Anne-Lise ; Kinzy, Willy ; Kneuer, Rainer ; Neumann, Ulf ; Rudin, Markus ; Staunfenbiel, Matthias ; Stoeckli, Markus ; Wiederhold, Karl-Heinz ; Gremlich, Hans-Ulrich

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 23 no. 5 (Mei 2005), page 577-583.
Topik: IMAGING; in vivo; imaging; oxazine-derivative probe

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.3 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

As Alzheimer's disease pathogenesis is associated with the formation of insoluble aggregates of amyloif b-peptide, approaches allowing the direct, non invasive visualization of plaque growth in vivo would be beneficial for biomedical research. Here we describe the synthesis and characterization of the near-infrared fluorescence oxzine dye AO1I987, which readily penetrates the intact blood brain barrier and binds to amyloid plaques. Using tenar infrared fluorescence imaging, we demonstrated specific interaction of AOI987 with amyloid plaques in APP23 transgenic mice in vivo, as confirmed by postmortem analysis of brain slices. Quantitative analysis revealed increasing fluorescence signal intensity with increasing plaque load of the animals and significant binding of AOI987 was observed for APP23 transgenic mice aged 9 months and older. Thus, AOI987 is an attractive probe to non invasively monitor disease progression in animal models of alzheimer disease and to evaluate effects of potential alzheimer disease drugs on the plaque load.

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