Systematic Interpretation of Genetic Interactions Using Protein Networks  

Oleh:

Kelley, Ryan ; Ideker, Trey

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 23 no. 5 (Mei 2005), page 561-566.
Topik: GENETICS; systematic; genetic; protein networks

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.3 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Genetic interaction analysis, in which two mutations have a combined effect not exhibited by either mutation alone, is a powerful and widespread tool for establishing functional linkages between genes. In the yeast sacharomyces verevisiae, on going screens have generated > 4,800 such genetic interaction data. We demonstrate that by combining these data with indormation on protein-protein, protein-DNA or metabolic networks, it is possible to uncover physical mechanisms behind many of the observed genetic effects. Using a probabilistic model, we found that 1,922 genetic interactions are significantly associated with either between or within pathway explanations encoded in the physical networks, covering -40% of known genetic interactions. These models predict new functions for 343 proteins and suggest that between pathway explanations are better than within pathway explanations at interpreting genetic interactions identified in systematic screens. This study provides a road map for how genetic and physical interactions can be integrated to reveal pathway organization and function.

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali