In Vivo Imaging Platform for Tracking Immunotherapeutic Cells  

Oleh:

Ahrens, Eric T. ; Flores, Rafael ; Hongyan Xu ; Morel, Penelope A.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 23 no. 8 (Agu. 2005), page 983-987.
Topik: cells; imaging platform; immunotherapeutic cells

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.4 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Cellular therapeutics show great promise for the treatment of diseases, but few noninvasive techniques exist for monitoring the cells after administration. Here we present a magnetic resonance imaging (MRI) technology that uses perfluoropolyether (PFPE) agents to track cells in vivo. Fluorine MRI selectively images only the labeled cells, and a conventional. H image places the cells in their anatomical context. We labeled phenotypically defined denritic cells (DCs) with PFPE ex vivo and observed efficient intracellular uptake of the PFPE with little effect on DC functions. We injected labeled DCs into tissue or intravemously in mice and then tracked the cells in vivo using F MRI. Although we focused on DCs, which are being developed as immunotherapeutics for cancer and autoimmune diseases, this technology should be useful for monitoring a wide range of cell types in vivo.

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