Coordinate Dual - Gene Transgenesis by Lentiviral Vectors Carrying Synthetic Bidirectional Promoters  

Oleh:

Amendola, Mario ; Venneri, Mary Anna ; Biffi, Alessandra ; Vigna, Elisa ; Naldini, Luigi

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 23 no. 1 (Jan. 2005), page 108-116.
Topik: vectors; gene transgenesis; lentiviral vestors; bidirectional promoters

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.2 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Transferring multiple genes into the same cell allows for the combination of genetic correction, marking, selection and confictional elimination of transduced cells or the reconstitution of multisubunit components and synergistic pathways. However this cannot be reliably accomplished by current gene transfer technologes. Based on the finding that some cellular promoters intrinsically promote divergent transcription, we have developed synthetic bidirectional promoters that mediate coordinate transcription of two mRNAs in a ubiquitous or a tissue specific manner. Lentivical vectors incorporating the new promoters enabled efficient dual gene transfer in several tissues in vivo after direct delivery or transgenesis and in a human gene therapy model. Because divergent gene pairs, likely trancribed from shared promotors, are common in the genome, the synthetic promoters that we developed may mimic a well represented feature of transcription. Vectors incorporating these promoters should increase the power of gene function studies and expand the reach and safety of gene therapy.

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