Microfluidic Sorting of Mammalian Cells by Optical Force Switching  

Oleh:

Wang, Mark M. ; Tu, Eugene ; Raymond, Daniel E. ; Joo Mo Yang ; Zhang, Haichuan ; Norbert, Hagen ; Bob, Dees ; Mercer, Elinore M. ; Forster, Anita H. ; Kariv, Ilona ; Marchand, Philippe J. ; Butler, William F.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 23 no. 1 (Jan. 2005), page 83-87.
Topik: switching; microfluidic; mammalian cells; optical force switching

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.2 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Microfluidic based devices have allowed miniaturization and increased parallelism of many common function in biological assays, however development of a practical technology for microfluidic based fluorescence activated cell sorting has proved challenging. Although a variety of different physical on chip switch mechanisms have been proposed, none has satisfied simultaneously the requirements of high throughput purity and recovery of live, unstressed mammalian cells. Here we show that optical forces can be used for the rapid (2-4 ms), active control of cell routing on a microfluidic chip. Optical switch controls reduce the complexity of the chip and simplify connnectivity. Using all optical switching we have implemented a fluorescence activated microfluidic cell sorter and evaluated its performance on live, stably transfected HeLa cells expressing a fused histone green fluorescent protein. Recovered populations were verified to be both viable and unstressed by evaluation of the transcriptional expression of two genes. HSPA6 and FOS, known indicators of cellular stress.

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