A Reverse Genetic Non - Transgenic Approach to Wheat Crop Improvement by Tilling  

Oleh:

Slade, Ann J. ; Fuerstenberg, Susan I. ; Loeffler, Dayna ; Steine, Michael N. ; Facciotti, Daniel

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 23 no. 1 (Jan. 2005), page 75-82.
Topik: GENETICS; reverse genetic. nontransgenic approach; TILLING

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.2 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

We report the use of TILLING (targeting induced local lesions in genomes), a revese genetic, nontransgenic method, to improve a quality trait in a polyploid crop plant. Waxy starches, composed mostly of amylopectin, have unique physiochemical properties. Wheat with only one or two functional waxy genes (granule bound starch synthase I, or GBSSI) produces starch with intermediate levels of amylopectin. We have identified 246 alleles of the waxy genes by TILLING each homoeolog in 1,920 allohexaploid and allotetraploid wheat individuals. These alleles encode waxy enzymes ranging in activity from near wild type to null, and they represent more genetic diversity than had been described in the preceding 25 years. A line of bread wheat containing homozygous mutations in two waxy homoeologs created through TILLING and a pre-existing deletion of the third waxy homoeolog displays a near null waxy phenotype. This approach to creating and identifying genetic variation shows potential as a tool for crop improvement.

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