Ex Vivo Generation of Fully Mature Human Red Blood Cells from Hematopoietic Stem Cells  

Oleh:

Giarratana, Marie-Catherine ; Kobari, Ladan ; Lapillonne, Helene ; Chalmers, David ; Kiger, Laurent ; Cynober, Therese ; Marden, Michael C. ; Wajcman, Henri ; Douay, Luc

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 23 no. 1 (Jan. 2005), page 69-74.
Topik: STEM CELL; red blood cells; hematopoietic; stem cell

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.2 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

We describe here the large scale ex vio production of mature human red blood cells (RBCs) from hematopoietic stem cells of diverse origins. By mimicking the marrow micro environment through the application of cytokines and co-cultuture on stromal cells, we coupled substantial amplification of CD34+ stem cells (up to 1,95 x 10 (6) - fold) with 100% terminal defferentiation into fully mature, functional RBCs. These cells survived in nonobese diabetic severe combined immuno deficient mice, as do native RBCs. Our sytem for producing cultured RBCs lends itself to a fundamental analysis of erythropoiesis and provides a simple in vitro model for studying important human viral or parasitic infections that target erythroid cells. Further development of large scale production of cultured RBCs will have implications for gene therapy, blood transfusion and tropical medicine.

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