The Genome Sequence of The Ethanologenic Bacterium Zymomonas Mobilis ZM4  

Oleh:

Jeong-Sun Seo ; Hyonyong Chong ; Hyun Seok Park ; Kyoung-Oh Yoon ; Cholhee Jung ; Jae Joon Kim ; Jin Han Hong ; Hyungtae Kim ; Jeong-Hyun Kim ; Joon-Il Kil ; Cheol Ju Park ; Hyun-Myung Oh ; Lee, Jung Soo ; Su-Jung, Jin ; Hye-Won Um ; Hee-Jong Lee ; Soo-Jin, Oh ; Jae Young Kim ; Hyung Lyun Kang ; Lee, Se Yong ; Lee, Kye Joon ; Hyen Sam Kang

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 23 no. 1 (Jan. 2005), page 63-68.
Topik: genome; genome sequence; ethanologenic bacterium

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.2 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

We report the complete genome sequence of zymomonas mobilis ZM4 (ATCC31821), an ethanologenic microorganism of interest for the production of fuel ethanol. The genome consists of 2,056,416 base pairs forming a circular chromosome with 1,998 open reading frames (OFRs) and three ribosomal RNA transcription units. The genome lacks recognizable genes for 6-phosphofructokinase, an essential enzyme of the embeden meyer of parnas pathway, and for two enzymes in the tricarboxylic acid cycle, the 2-oxoglutarate dehydrogenase complex and malate dehydrogenase, so glucose can be metabolized only by the entner-doudoroff pathway. Whole genome microarrays were used for genomic comparisons with the Z mobilis type strain ZM1 (ATCC10988) revealing that 54 ORFs predicted to encode for transport and secretory proteins, transcriptional regulators and oxidoreductase in the ZM4 strain were absent from ZM1. Most of these ORFs were also found to be actively transcribed in association with ethanol production by ZM4.

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