Specification of Motoneurons from Human Embryonic Stem Cells  

Oleh:

Xue-Jun, Li ; Zhong-Wei, Du ; Zarnowska, Ewa D. ; Pankratz, Matthew ; Hansen, Lauren O. ; Pearce, Robert A. ; Su-Chun, Zhang

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 23 no. 2 (Feb. 2005), page 215-221.
Topik: STEM CELL; motoneurons; human embryonic; stem cell

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.2 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

An understanding of how mamalian stem cells produce specific neuronal subtypes remains exclusive. Here we show that human embryonic stem cells generated early neuroectodermal cells, which organized into rosettes and expressed Pax6 but not Sox1, and the late neuroectodermal cells, which formed neural tube like structures and expressed both pax6 and Sox1. Only the early, but not the late, neuroectodermal cells weer efficiently posteriorized by retinoic acid and in the presence of sonic hedgehod, differentiated into spinal motoneurons. The in vitro generated motoneurons expressed HB9, HoxC8, choline acetyltransferase and vesicular acetylcholine transporter, induced clustering of actylcholine receptors in myotubes, and were electro physiologically active. These findings indicate that retinoic acid action is required during neuroectoderm induction for motoneuron specification and suggest that stem cells have restricted capacity to generate region specific projection neurons even at an early developmental stage.

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