Effect of Sampling on Topology Predictions of Protein - Protein Interaction Networks  

Oleh:

Jing-Dong J. Han ; Dupuy, Denis ; Bertin, Nicolas ; Cusick, Michael E. ; Vidal, Marc

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 23 no. 7 (Jul. 2005), page 839-844.
Topik: TOPOLOGY; topology; interaction netwos

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.3 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Currently available protein-protein interaction (PPI) network of interactome maps, obtained with the yeast two hybrid (Y2H) assay or by co-affinity purification followed by mass spectrometry (co-AP/MS) only cover a fraction of the complete PPI networks. These partial networks display scale free topologies, most proteins participate in only a few interactions whereas a few proteins have many interaction partners. Here we analyze whether the scale free topologies of the partial networks obtained from Y2H assays can be used to accurately under the topology of complete interactomes. We generated four theoretical interaction networks of different topologies (random, exponential power law, truncated normal) partial sampling of these networks resulted in sub networks with topological characteristics that were virtually indistinguishable from those of currently available Y2H derived partial interactome maps. We conclude that given the current limited coverage levels, the observed scale free topology of existing interactome maps cannot be conficently extrapolated to complete interactomes.

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