Analysis of The Transcriptional Complexity of Arabidopsis Thaliana by Massively Parallel Signature Sequencing  

Oleh:

Meyers, Blake C. ; Vu, Tam H. ; Tej, Shivakundan Singh ; Ghazal, Hassan ; Matvienko, Marta ; Agrawal, Vikas ; Jianchang Ning ; Haudenschild, Christian D.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 22 no. 8 (Agu. 2004), page 1006-1012.
Topik: SEQUENCE ANALYSIS-METHODS; transcriptional complexity; signature sequencing

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.1 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Large scale sequencing of short mRNA derived tags can establish the qualitative and quantitative characteristics of a complex transcriptome. We sequenced 12,304,362 thats from five diverse libraries of arabidopsis thaliana using massively parallel signature sequencing (MPSS). A tota of 48,572 distinct signatures, each representing a different transcript, were expressed at significant levels. These signature were compared to the annotation of the A. Thaliana genomic sequence, in the five libraries, this comparison yielded between 17.353 and 18,361 genes with sense ecoerssion, and between 5,487 and 8m729 genes with antisense expression. An additional 6,691 MPSS signatures mapped to unannotated regions of the genome. Expression was demonstrated for 1,168 genes for which expression data were previously unknown. Alternative polydeylation was observed for more than 25% of A. thaliana genes transcribed in these libraries. The MPSS expression data suggest that the A. thaliana transcriptome is complex and contain many as yet uncharacterized variants of normal coding transcripts.

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali