Systematic Identification of Abundant A to I Editing Sites in The Human Transcriptome  

Oleh:

Levanon, Erez Y. ; Eisenberg, Eli ; Yelin, Rodrigo ; Nemzer, Sergey ; Hallegger, Martina ; Shemesh, Ronen ; Fligelman, Zipora Y. ; Shoshan, Avi ; Pollock, Sarah R. ; Sztybel, Dan ; Olshansky, Moshe ; Rechavi, Gideon ; Jantsch, Michael F.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 22 no. 8 (Agu. 2004), page 1001-1005.
Topik: human; abundant; editing; human transcriptome

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.1 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

RNA editing by members of the ADAR (adenosine deaminases acting on RNA) family leads to sitre specific conversion of adenosine to inosine (A to I) in precursor messenger RNAs. Editing by ADARs is believed to occur in all metazoa and is essential for mammalian development. Currently, only a limited number of human ADAR substrates are known, whereas indirect evidence suggests a substantial fraction of all pre-mRNAs being affected. Here we describe a computational search for ADAR editing sites in the human transcriptome using millions of available expressed sequences. We mapped 12,723 A to I editing sites in 1,637 different genes, with an estimated accuracy of 95% raising the number of known editing sites by two orders of magnitude. We experimentally validated our method by verifying the occurence of editing in 26 novel substrates. A to I editing in humans primarily occurs in non coding regions of the RNA, typically in Alu repeats. Analysis of the large set of editing sites indicates the role of editing in controlling dsRNA stability.

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