Direct Proteomic Mapping of The Lung Microvascular Enfothelual Cell Surface in Vivo and in Cell Culture  

Oleh:

Durr, Eberhard ; Jingyi Yu ; Krasinska, Karolina M. ; Carver, Lucy A, ; Yates, John R. ; Testa, Jacqueline E. ; Oh, Phil ; Schnitzer, Jan E.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 22 no. 8 (Agu. 2004), page 985-992.
Topik: proteomics; proteomic; lung microvascular; endothelial cell surface; culture

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.1 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Endothelial cells can function differently in vitro and in vivo, however, the degree of micro environmental modulation in vivo remains unknown at the molecular level largerly because of analytical limitations. We use multidimensional protein identification technology (MudPIT) to identify 450 proteins with three or more spectra) in luminal endothelial cell plasma membranes isolated from rat lungs and from cultured rat lung microvascular endothelial cells. Forty one percent of proteins expressed in vivo are not detected in vitro. Statistical analysis measuring reproducibility reveals that seven to ten MudPIT measurements are necessary to achieve > 95% confidence of analytical completeness with current ion trap equipment. large-scale mapping of the proteome of vascular endothlial cell surface in vivo, as demonstrated here, is advisable because distintc protein expression is apparently regulated by the tissue micro environment that cannot yet be duplicated in standard cell culture.

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali