Gas - Inducible Transgene Expression in Mammalian Cells and Mice  

Oleh:

Baba, Marie Daoud-El ; Aubel, Dominique ; Weber, Wilfried ; Fussenegger, Martin ; Rimann, Markus ; Spielmann, Manuela ; Keller, Bettina ; Weber, Cornelia C.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 22 no. 11 (Nov. 2004), page 1440-1444.
Topik: cells; gas-inducible; transgene expression; mammalia cells; mice

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.3 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

We describe the design and detailed characterization of a gas-inducible transgene control system functional in different mammalian cells, mice and prototype biopharmaceutical manufacturing. The acetaldehyde inducible AlcR-P (alcA) transactivator promoter interaction of the aspergillus nidulans ethanol catablizing regulon was engineered for gas adjustable transgene expression in mammalian cells. Fungal AlcR retained its transactivation characteristic in a variety of mammalian cell lines and reversible adjusted transgene transcription from chimeric mammalian promoters (P air) containing P (alcA) derived operators in a gaseous acealdehyde dependent manner mice implanted with microencapsulated cells engineered for acetaldehyde inducible regulation (AIR) of the human glycoprotein secreted placental alkaline phosphatase showed adjustable serum phosphatase levels after exposure to different gaseous acetaldyehyde concentrations AIR controlled interferon (b) production in transgenic CHO-K1 derived serum free suspension cultures could be modulated by fine tuning inflow and outflow of acetaldehyde containing gas during standard bioreactor operation. AIR technology could serve as a tool for therapeutic transgene dosing as well as biopharmaceutical manufacturing.

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