Advances in The Production of Human Therapeutic Proteins in Yeasts and Filamentous Fungi  

Oleh:

Gerngross, Tillman U.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 22 no. 11 (Nov. 2004), page 1409-1414.
Topik: PROTEIN; human theurapeutic protein; yeasts; filamentous fungi

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.3 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Yeast and fungal protein expression systems are used for the production of many industrially relevant enzymes, and are widely used by the research community to produce proteins that cannot be actively expressed in Escherichia coli or require glycosylation for proper folding and biological activity. However, for the production of theurapeutic glycoproteins intended for use in humans, yeasts have been less useful because of their inability to modify proteins with human glycosylation structures. Yeast glycosylation is of the high mannose type, which confers a short in vivo half life to the protein and may render it less efficacious or even immonogenic. Several ways of humanizing yeast derived glycoproteins have been tried, including enzymatically modifying proteins in vitro and modulating host glycosylation pathways in vivo. Recent advances in the glycoengineering of yeasts and the expression of theurapeutic glycoproteins in humanized yeasts have shown significant promise, and are challenging the current dominance of theurapeutic protein production based on mammalian cell culture.

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