Homogeneous Detection of Unamplified Genomic DNA Sequences Based on Colorimetric Scatter of Gold Nanoparticle Probes  

Oleh:

Storhoff, James J. ; Lucas, Adam D. ; Garimella, Viswanadham ; Bao, Y. Paul ; Muller, Uwe R.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 22 no. 7 (Jul. 2004), page 883-887.
Topik: dna; homogeneous detection; unamplified genomic; DNA sequnces; colorimetric scatter; gold nanoparticles probes

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.1 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Nucleid Acid Diagnostics is dominate by fluorescence based assays that use complex and expensive enzyme based target or signal amplification procedures. many clinical diagnostic applications will require simpler, inexpensive assays that can be done in a screening mode. We have developed a spot and read colorimetric detection method for identifying nucleic acid sequences based on the distance dependent optical properties of gold nanoparticles. In this assay, nucleid acid targets are recognized by DNA modified gold probes, which undergo a color change that is visually detectable when the solutions are spotted onto an illuminated glass waveguide. This scatter based method enables detection of zeptomole quantities of nucleic acid targets without target or signal amplification when coupled to an improved hybridization method that facilitates probe target binding in a homogeneous format. In comparison to previously reported absorbance based method, this method increases detection sensitivity by over four orders of magnitude. We have applied thies by over four orders of magnitude. We have applied this method to the rapid detection of mecA in methicillin resistant Staphylococcus aureus genomic DNA samples.

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