Single - Chain Antibodies Against a Plant Viral RNA Dependent RNA Polymerase Confer Virus Resistance  

Oleh:

Boonrod, KaJohn ; Galetzka, Danuta ; Nagy, Peter D. ; Conrad, Udo ; Krczal, Gabi

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 22 no. 7 (Jul. 2004), page 856-862.
Topik: antibodies; single chain antibodies; plant viral RNA; RNA polymerase; virus resistance

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.1 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Crop loss due to viral diseases is still a major problem for agriculture today. We present a strategy to achieve virus resistance based on the expression of single chain Fv fragments (scFvs) against a conserved domain in a plant viral RNA - dependent RMNA polymerase (RdRp), a key enzyme in virus replication. The selected scFvs inhibited complementary RNA synthesis of different plant virus RdRps in vitro and virus replication in planta. Moreover, the scFvs also bound to the RdRp of the distantly related hepatitis C virus. T1 and T2 progeny of transgenic lines of Nicotiana benthamiana expressing different scFvs either in the cytosol or in the endoplasmic reticulum showed varying degrees of resistance against four plant viruses from different genera, three of which belong to the tombusviridae family. Virus resistance based on antibodies to RdRps adds another tool to the repertoire for combating plant viruses.

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