The Ins and Outs of Gene Expression Control  

Oleh:

Perler, Francine B.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 22 no. 7 (Jul. 2004), page 824-825.
Topik: EXPRESSION; ins and outs; experssion control

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.1 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Tempertaure sensitive mutant inteins inserted within two transcription factors provide a new tool for controlling gene expression in yeast and flies. Temperature sensitive mutants have been a mainstay of genetic analysis since the early 20th century. But often such mutants can be difficult to isolate for a protein of interest and, if found, the amino acid changes introduced by the mutation may compromise function. In this issue, Zeidler er al, combine temperature sensitive (TS) inteins protein elements capable of slf excision from a precursor protein and ligation of the flanking protein regions with the yeast transcription factors Gal4 and Gal80 to create a system that overcomes these problems. For cells containing genes under the control of the Gal4 promoter, a simple temperature shift activates protein splicing of hybrid Gal4 and Gal80, resulting in excision of the intein and restoration of transcription factor functionality....

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