Allotopic Expression of a Gene Encoding FLAG Tagged-Subunit 8 of Yeast Mitochondrial ATP Synthase  

Oleh:

Artika, I Made

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Hayati Journal of Biosciences vol. 13 no. 1 (Mar. 2006), page 36-38.
Topik: Yeast; Allotopic Expression; Mitochondria; ATP Synthase
Fulltext: 69.pdf (60.79KB)

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Perpustakaan Pusat (Semanggi) Nomor Panggil: HH13.8 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Subunit 8 of yeast mitochondrial ATP synthase is a polypeptide of 48 amino acids encoded by the mitochondrial ATP8 gene. A nuclear version of subunit 8 gene has been designed to encode FLAG tagged-subunit 8 fused with a mitochondrial signal peptide. The gene has been cloned into a yeast expression vector and then expressed in a yeast strain lacking endogenous subunit 8. Results showed that the gene was successfully expressed and the synthesized FLAG taggedsubunit 8 protein was imported into mitochondria. Following import, the FLAG tagged-subunit 8 protein assembled into functional mitochondrial ATP synthase complex. Furthermore, the subunit 8 protein could be detected using anti-FLAG tag monoclonal antibody.

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