Daya Regenerasi Padi Indica CV Bengawan Solo Salam Dua Tipe Media Regenerasi dengan Penembakan Mikroproyektil  

Oleh:

Sudarsono ; Hajrial, Aswidinnoor ; Joko, Santoso Tri ; Hanarida, Somantri Ida

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Hayati Journal of Biosciences vol. 12 no. 4 (Dec. 2005), page 157-161.
Topik: PADI; Padi Indica; Penembakan Mikroproyektil
Fulltext: 59.pdf (68.32KB)

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: HH13.7 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Factors affecting the success of gene delivery into cells/tissues of rice mediated by particle bombardment have been investigated. However, their effects on regeneration capacity of rice explants have not been investigated. Objectives of this experiments were to evaluate the effects of particle bombardment on regeneration capacity of indica rice cv. Bengawan Solo calli and the effects of four regeneration media on the development of bombardment calli. Calli of indica rice were initiated from mature seeds on callus-inducing medium. They were transferred on osmoticum containing medium prior to bombardment and maintained on the medium up to two days. Particle bombardment was conducted at 16cm bombardment distance, 27cm Hg helium pressure and using standard procedures for preparation of microprojectile particles. Results of the ecperiment indicated particle bombardment affected the durvival of explants of indica rice but did not affect the development of the explants. The development if explants was rather then dependent on the regeneration media than on the particle bombardment treatment. RMS medium was better than RNB medium for shoots inducing from bombarded indica rice explants.

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